microscopy and analysis Correlative Light and Electron Microscopy
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چکیده
منابع مشابه
Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasib...
متن کاملHigh Data Output and Automated 3D Correlative Light–Electron Microscopy Method
Correlative light/electron microscopy (CLEM) allows the simultaneous observation of a given subcellular structure by fluorescence light microscopy (FLM) and electron microscopy. The use of this approach is becoming increasingly frequent in cell biology. In this study, we report on a new high data output CLEM method based on the use of cryosections. We successfully applied the method to analyze ...
متن کاملCorrelative cryo-fluorescence light microscopy and cryo-electron tomography of Streptomyces.
Light microscopy and electron microscopy are complementary techniques that in a correlative approach enable identification and targeting of fluorescently labeled structures in situ for three-dimensional imaging at nanometer resolution. Correlative imaging allows electron microscopic images to be positioned in a broader temporal and spatial context. We employed cryo-correlative light and electro...
متن کاملCorrelative fluorescence and electron microscopy on ultrathin cryosections: bridging the resolution gap.
Microscopy has become increasingly important for analysis of cells and cell function in recent years. This is due in large part to advances in light microscopy that facilitate quantitative studies and improve imaging of living cells. Analysis of fluorescence signals has often been a key feature in these advances. Such studies involve a number of techniques, including imaging of fluorescently la...
متن کاملA Streamlined Technique to Examine Cell Monolayers by means of Correlative Light and Transmission Electron Microscopy
Correlative light/electron microscopy (CLEM) combines the power of light microscopy to search large areas for fluorescently-tagged live cells/regions of interest and transmission electron microscopy (TEM) to image the same specimen at high magnification for ultrastructural characterization. CLEM methods are essential to observe dynamic processes such as organelle biogenesis and intracellular tr...
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تاریخ انتشار 2013